gastro-jРоссийский журнал гастроэнтерологии, гепатологии, колопроктологииRussian Journal of Gastroenterology, Hepatology, Coloproctology1382-43762658-6673«Gastro» LLCgastro-j-1016Research ArticleГЕПАТОЛОГИЯHEPATOLOGYГипергомосистеинемия, гиперцистеинемия, глутаматная эксайтотоксичность, дефицит таурина при гепатите СHyperhomocisteinemia, hypercysteinemia, glutamate excitotoxicity, taurine deficiency at hepatitis CПоздеевВ. К.PozdeevV. K.

Поздеев Владимир Константинович – доктор медицинских наук, профессор по специальности биохимия, ведущий научный сотрудник отделения экспериментально-клинических исследований

197376, г. Санкт-Петербург, ул. Профессора Попова, д. 15/17

Pozdeev Vladimir K – MD, professor of biochemistry, research associate, Division of clinical and experimental investigations

197376, Saint-Petersburg, Prof. Popova street, 15/17

vkpozdeev@mail.ruПоздеевН. В.PozdeevN. V.

Поздеев Никита Владимирович – кандидат биологических наук, врач-эндокринолог

MS 8106, 12801 E. 17th Ave, RC1South, 7th floor room 7103, Aurora, CO 80045

Pozdeyev Nikita V – MD, PhD, endocrine fellow

MS 8106, 12801 E. 17th Ave, RC1-South, 7th floor room 7103, Aurora, CO 80045

nikitapozdeyev@gmail.comНикитинаО. Е.NikitinaO. Ye.

Никитина Олеся Евгеньевна – кандидат медицинских наук, старший научный сотрудник отделения экспериментально-клинических исследований

197376, г. Санкт-Петербург, ул. Профессора Попова, д. 15/17

Nikitina Olesya Ye – PhD, senior researcher, Division of clinical and experimental investigations

197376, Saint-Petersburg, Prof. Popova street, 15/17

nikitina@influenza.spb.ruФГБУ «НИИ гриппа» Минздрава РоссииРоссияFederal government-financed institution «Research Institute of Influenza», Ministry of healthcare of the Russian FederationRussian FederationДепартамент медицины Университета штата КолорадоСоединённые Штаты АмерикиDepartment of Medicine, Colorado State UniversityUnited States2015180720152534960Copyright © Поздеев В.К., Поздеев Н.В., Никитина О.Е., 20152015Поздеев В.К., Поздеев Н.В., Никитина О.Е.Pozdeev V.K., Pozdeev N.V., Nikitina O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.gastro-j.ru/jour/article/view/1016

Цель исследования. Оценка метаболических нарушений, вызванных хроническим течением гепатита С.Материал и методы. В плазме крови 55 больных гепатитом С и 14 здоровых волонтеров методом высокоэффективной жидкостной хроматографии исследованы уровни общего гомоцистеина (tHCy), общего цистеина (tCys), общего глутатиона (tGSH) и свободных аминокислот (Asp, Asn, Glu, Gln, Tau, Ser).Результаты. При тотальной статистической обработке данных при гепатите С наблюдаются: гипергомоцистеинемия (содержание tHCy повышено в 2,5 раза), гиперцистеинемия (содержание tCys повышено в 1,5 раза), глутаматная эксайтотоксичность (содержание Glu повышено в 2,75 раза на фоне относительного снижения уровней tGSH и Gln), дефицит таурина (его содержание снижено в 1,4 раза), увеличение содержания Gln, Asp, Asn, Ser в 1,56, 1,75, 1,32, 1,4 раза соответственно. Обработка данных путем построения полигонов распределения частот по уровням содержания веществ в плазме крови больных гепатитом С выявила гетерогенность патохимии гепатита С по tGSH, tCys и Gln; однако в совокупностях как с относительно низким, так и высоким уровнями этих веществ содержание tHCy, Glu и Asp остается повышенным и отражает явление гипергомоцистеинемии и глутаматной эксайтотоксичности. У пациентов с высоким содержанием в крови tGSH и Gln глутаматная эксайтотоксичность выражена меньше — уровни tHCy, Glu и Asp повышены в меньшей степени, отмечается тесная прямая корреляционная зависимость между tGSH, Gln, tCys и Ser.Заключение. Метаболическая терапия может существенно снизить негативное влияние метаболических нарушений и повысить эффективность применения ПЭГ-ИФН-α в сочетании с рибавирином для лечения гепатита С.

Aim of investigation

Aim of investigation. An estimation of metabolic disorders related to chronic course of hepatitis C.

Material and methods

Material and methods. Total homocysteine (tHCy), total cysteine (tCys), total glutathione (tGSH) and free aminoacids (Asp, Asn, Glu, Gln, Tau, Ser) levels were measured by high-performance liquid chromatography in plasma of 55 hepatitis C patients and 14 healthy volunteers.

Results

Results. Total statistical data processing at hepatitis C revealed: hyperhomocisteinemia (2,5 times increase in tHCy level), hypercysteinemia (1,5 times increase in tCys level), glutamate excitotoxicity (2,75 times increase in Glu level on a background of relative decrease of levels of tGSH and Gln), deficiency of taurine (level was decreased 1,4 times), increase of Gln, Asp, Asn, Ser levels 1,56, 1,75, 1,32, 1,4 times respectively. Data processing by nonnormal histogram plotting of substances plasma levels in hepatitis C patients revealed heterogeneity of pathochemistry of hepatitis C for tGSH, tCys and Gln. However in sets both with relatively low and high levels of these substances content of tHCy, Glu and Asp remains increased and reflects hyperhomocisteinemia and glutamate excitotoxicity phenomena. In patients with high content of tGSH and Gln in blood glutamate excitotoxicity was less expressed — levels of tHCy, Glu and Asp were increased to lesser degree, strong positive correlation between tGSH, Gln, tCys and Ser was marked.

Conclusion

Conclusion. Metabolic therapy can essentially decrease negative effect of metabolic disorders and increase efficacy of PEG-IFN-α in combination to ribavirin at hepatitis C treatment.

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The authors declare that there are no conflicts of interest present.