gastro-jРоссийский журнал гастроэнтерологии, гепатологии, колопроктологииRussian Journal of Gastroenterology, Hepatology, Coloproctology1382-43762658-6673«Gastro» LLC10.22416/1382-4376-2017-27-3-27-33gastro-j-155Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL ARTICLESРоль полиморфизмов генов цитокинов в развитии острого панкреатита: анализ межгенных и генно-средовых взаимодействийThe role of cytokine genetic polymorphism in development of acute pancreatitis: analysis of intergenic and environmental interactionsСамгинаТ. А.SamginaT. A.tass@list.ruЖивотоваГ. А.ZhivotovaG. A.noemail@neicon.ruНазаренкоП. М.NazarenkoP. M.noemail@neicon.ruПолониковА. В.PolonikovA. V.noemail@neicon.ruФГБОУ ВО «Курский государственный медицинский университет»РоссияFederal state educational government-financed institution of higher education «Kursk state medical university»Russian Federation2017120820182732733Copyright © Самгина Т.А., Животова Г.А., Назаренко П.М., Полоников А.В., 20182018Самгина Т.А., Животова Г.А., Назаренко П.М., Полоников А.В.Samgina T.A., Zhivotova G.A., Nazarenko P.M., Polonikov A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.gastro-j.ru/jour/article/view/155

Цель исследования. Изучить связь полиморфизмов (rs16944) гена интерлейкина-1 (IL-1), (rs1800795) гена интерлейкина-6 (IL-6), (rs1800896) гена интерлейкина-10 (IL-10) с развитием острого панкреатита (ОП) в русской популяции. Материал и методы. Образцы цельной крови были получены у 297 больных ОП и 238 здоровых лиц. Генотипирование полиморфизмов (rs16944) гена IL-1, (rs1800795) гена IL-6, (rs1800896) гена IL-10 выполнено с использованием полимеразной цепной реакции путем дискриминации аллелей с помощью TaqMan-зондов. Результаты. Установлено, что комбинация генотипов полиморфизмов 511СТ×174GC генов IL-1 и IL-6 ассоциирована с риском развития ОП (OR=2,25, 95% CI 1,45-3,49; p=0,0018). Проведенный стратифицированный анализ показал, что у курящих пациентов с генотипом 511СТ риск развития ОП выше, чем у пациентов с другими генотипами (OR=2,22, 95% CI 1,3-3,79; p=0,003). Согласно результатам анализа парных сочетаний генотипов исследуемых генов с риском развития заболевания, у пациентов с комбинацией генотипов 511СT×174GС риск развития ОП повышен при алкогольном анамнезе более 10 лет (OR=2,88, 95% CI 1,59-5,23; p=0,0004). Заключение. Исследование генетических полиморфизмов интерлейкинов может оказаться полезным для оценки цитокинового статуса пациентов в процессе течения ОП с целью прогнозирования его исходов и разработки персонализированных подходов к лечению и профилактике заболевания.

Aim of investigation. To study association interleukin-1 (IL-1) gene (rs16944), interleukin-6 (IL-6) gene (rs1800795), interleukin-10 (IL-10) gene (rs1800896) polymorphisms with development of acute pancreatitis (AP) in the Russian population. Material and methods. Whole blood samples were received from 297 AP patients and 238 healthy controls. Genotyping of IL-1 gene (rs16944) polymorphisms, IL-6 gene (rs1800795), IL-10 gene (rs1800896) was carried out by polymerase chain reaction with allele discrimination by TaqMan-probes. Results. The genetic polymorphism combination 511СТ×174GC of IL-1 and IL-6 genes was associated to high risk of AP development (OR=2.25, 95%-CI 1.45-3.49; p=0.0018). According to stratification analysis smoking patients with 511CT genotype had higher AP risk, then the patients with other genotypes (OR=2.22, 95%-CI 1.3-3.79; p=0.003). Paired combination of genotypes to disease risk analysis demonstrated that at 511СT×174GС genotype combination the AP risk is highest at alcohol abuse history for over 10 years (OR=2.88, 95%-CI 1.59-5.23; p=0.0004). Conclusion. Interleukin genetic polymorphism investigation may be useful at assessment of cytokine status in AP patients to predict the outcomes and to develop the personalized approach to treatment and prophylaxis.

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The authors declare that there are no conflicts of interest present.