gastro-jРоссийский журнал гастроэнтерологии, гепатологии, колопроктологииRussian Journal of Gastroenterology, Hepatology, Coloproctology1382-43762658-6673«Gastro» LLCgastro-j-1775Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL ARTICLESОригинальный отечественный пробиотик аципол: молекулярно­-биологические и метаболические характеристикиOriginal Russian probiotic Acipol: molecular-biologic and metabolic characteristicsИвашкинаН. Ю.IvashkinaN. Yu.БотинаС. Г.BotinaS. G.2009050320091925864Copyright © Ивашкина Н.Ю., Ботина С.Г., 20092009Ивашкина Н.Ю., Ботина С.Г.Ivashkina N.Y., Botina S.G.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.gastro-j.ru/jour/article/view/1775

Цель исследования. Изучить молекулярно-­биологические и метаболические свойства препарата «Аципол» с учетом современных требований, предъявляемых к пробиотическим средствам.Материал и методы. Для изучения качественных свойств аципола проведено исследование штаммов препарата с помощью полимеразной цепной реакции (ПЦР) амплификации ДНК и секвенирования гена 16S РНК штаммов L. acidophilus NK1, NK2, NK5, NK12. Отсутствие мобильной геномной информации установлено при ультрацентрифугировании клеток бактерий с дальнейшим электрофоретическим разделением и визуализацией экстрахромосомной фракции в агарозном геле. Метаболические свойства штаммов аципола изучены с помощью стандартных биохимических и микробиологических методик.Результаты. Проведенное исследование доказало видовую принадлежность штаммов L. acidophilus NK1, NK2, NK5, NK12 на генетическом уровне, установлена способность штаммов синтезировать экзополисахариды, переносить низкие значения pH и воздействие желчи, выявлены свойства лактобактерий продуцировать витамины, подавлять рост условно­патогенной микрофлоры, проявлять устойчивость к метронидазолу.Выводы. Комплексная оценка аципола свидетельствует о его соответствии современным требованиям, предъявляемым к биопрепаратам.

Aim of investigation

Aim of investigation. To study molecular-biologic and metabolic properties of agent «Acipol» with account to modern requirements to probiotic drugs.

Materials and methods

Materials and methods. To study quality properties of Acipol investigation of strains of the agent by polymerase chain reaction (PCR) of DNA amplification and sequenation of 16S RNA gene of NK1, NK2, NK5, NK12 strains of L. acidophilus was carried out. At ultracentrifugation of bacterial cells with further electrophoretic separation and visualization of extrachromosomal fraction in agarose gel absence of mobile genomic information was found. Metabolic properties of Acipol strains were studied by standard biochemical and microbiologic procedures.

Results

Results. The original study proved specific belonging of NK1, NK2, NK5, NK12 strains of L. acidophilus at genetic level, ability of strains to synthesize exopolysaccharides, to tolerate low pH values and influence of bile. Properties of lactobacilli to produce vitamins, to suppress proliferation of saprophytic microflora, as well as metronidazole-resistance properties were revealed.

Conclusions

Conclusions. The complex assessment of Acipol testifies to its conformity to the up-to-date requirements to biological preparations.

АциполбиопрепаратыПЦРвидовая принадлежностьметаболические свойстваacipolbiological preparationsPCRspecific belongingmetabolic propertiesReferencesМатериалы круглого стола «Рациональный выбор пробиотика в практике гастроэнтеролога» (Москва, 18 декабря 2006 г.) // Рос. мед. вести. – 2007. – Т. 12, № 4. – С. 46–48.Материалы круглого стола «Рациональный выбор пробиотика в практике гастроэнтеролога» (Москва, 18 декабря 2006 г.) // Рос. мед. вести. – 2007. – Т. 12, № 4. – С. 46–48.Материалы научно-практической конференции «Probiotech 2007». – Нант, Франция, 11–12 июня 2007 г.Материалы научно-практической конференции «Probiotech 2007». – Нант, Франция, 11–12 июня 2007 г.Boyer T.G., Chen P.L., Lee W.H. Genome mining for human cancer genes: wherefore art thou? // Trends Mol. Med. – 2001. – Vol. 7, N 5. – P. 187–189.Boyer T.G., Chen P.L., Lee W.H. Genome mining for human cancer genes: wherefore art thou? // Trends Mol. Med. – 2001. – Vol. 7, N 5. – P. 187–189.Chakrabortty A., Das S., Majumdar S. et al. Use of RNA arbitrarily primed-PCR fingerprinting to identify Vibrio cholerae genes differentially expressed in the host following infection // Infect. Immun. – 2000. – Vol. 68. – P. 3878–3887.Chakrabortty A., Das S., Majumdar S. et al. Use of RNA arbitrarily primed-PCR fingerprinting to identify Vibrio cholerae genes differentially expressed in the host following infection // Infect. Immun. – 2000. – Vol. 68. – P. 3878–3887.D’Souza A.L., Rajkumar C., Cooke J., Bulpitt C.J. Probiotics in prevention of antibiotic associated diarrhea: meta-analysis // BMJ. – 2002. – Vol. 324, N 8. – P. 1350–1361.D’Souza A.L., Rajkumar C., Cooke J., Bulpitt C.J. Probiotics in prevention of antibiotic associated diarrhea: meta-analysis // BMJ. – 2002. – Vol. 324, N 8. – P. 1350–1361.Fuller R. Probiotics in man and animals // J. Appl. Bacteriol. – 1989. – Vol. 66, N 5. – P. 365–378.Fuller R. Probiotics in man and animals // J. Appl. Bacteriol. – 1989. – Vol. 66, N 5. – P. 365–378.Guandalini S., Pensabene L., Zikri M.A. et al. Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea: a multicenter European trial // J. Pediatr. Gastroenterol. Nutr. – 2000. – Vol. 30, N 1. – P. 54–60.Guandalini S., Pensabene L., Zikri M.A. et al. Lactobacillus GG administered in oral rehydration solution to children with acute diarrhea: a multicenter European trial // J. Pediatr. Gastroenterol. Nutr. – 2000. – Vol. 30, N 1. – P. 54–60.Guidelines for the evaluation of probiotics in food: Joint FAO/WHO Working Group meeting. – London, Ontario, Canada, 30 April–1 May 2002.Guidelines for the evaluation of probiotics in food: Joint FAO/WHO Working Group meeting. – London, Ontario, Canada, 30 April–1 May 2002.Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. – Cordoba, Argentina, 1–4 October 2001.Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. – Cordoba, Argentina, 1–4 October 2001.Leblond-Bourget N., Philippe H., Mangin I., Decaris B. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny // Int. J. Syst. Bacteriol. – 1996. – Vol. 46, N 1. – P. 102–111.Leblond-Bourget N., Philippe H., Mangin I., Decaris B. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny // Int. J. Syst. Bacteriol. – 1996. – Vol. 46, N 1. – P. 102–111.Lilly D.M., Stillwell R.H. Probiotics: growth-promoting factors produced by microorganisms // Science. – 1965. – N 12. – P. 747–748.Lilly D.M., Stillwell R.H. Probiotics: growth-promoting factors produced by microorganisms // Science. – 1965. – N 12. – P. 747–748.Lodinova-Zadnikova R., Cukrowska B., Tlaskalova-Hogenova H. Oral administration of probiotic Escherichia coli after birth reduces frequency of allergies and repeated infections later in life (after 10 and 20 years) // Int. Arch. Allergy Immunol. – 2003. – Vol. 131, N 3. – P. 209–211.Lodinova-Zadnikova R., Cukrowska B., Tlaskalova-Hogenova H. Oral administration of probiotic Escherichia coli after birth reduces frequency of allergies and repeated infections later in life (after 10 and 20 years) // Int. Arch. Allergy Immunol. – 2003. – Vol. 131, N 3. – P. 209–211.McFarland L.V. Meta-analysis of probiotics for the prevention of antibiotic associated diarrhea and the treatment of Clostridium difficile disease // Am. J. Gastroenterol. – 2006. – Vol. 101, N 4. – P. 812–822.McFarland L.V. Meta-analysis of probiotics for the prevention of antibiotic associated diarrhea and the treatment of Clostridium difficile disease // Am. J. Gastroenterol. – 2006. – Vol. 101, N 4. – P. 812–822.Rolfe R.D. The role of probiotic cultures in the control of gastrointestinal health // J. Nutr. – 2000. – Vol. 130, N 2 (suppl.). – P. 396–402.Rolfe R.D. The role of probiotic cultures in the control of gastrointestinal health // J. Nutr. – 2000. – Vol. 130, N 2 (suppl.). – P. 396–402.Stanton C., Gardiner G., Meehan H. et al. Market potential for probiotics // Am. J. Clin. Nutr. – 2001. – Vol. 73 (suppl. 2). – P. 476–483.Stanton C., Gardiner G., Meehan H. et al. Market potential for probiotics // Am. J. Clin. Nutr. – 2001. – Vol. 73 (suppl. 2). – P. 476–483.Torriani S., Zapparoli G., Dellaglio F. Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis // Appl. Environ. Microbiol. – 1999. – Vol. 65. – P. 4351–4356.Torriani S., Zapparoli G., Dellaglio F. Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis // Appl. Environ. Microbiol. – 1999. – Vol. 65. – P. 4351–4356.

The authors declare that there are no conflicts of interest present.