Colonic growth factors (G-CSF, GM-CSF) and chemokines (MCP-1, MIP-1β) in severe ulcerative colitis
https://doi.org/10.22416/1382-4376-2016-5-74-81
Abstract
Aim of investigation. To determine the content of growth factors (G-CSF, GM-CSF) and chemokines (MCP-1, MIP-1β) in resected colon specimens of patients with severe ulcerative colitis (UC). Material and methods. Overall 48 biopsy specimens of the colonic mucosa resected at 7 patients with total UC were investigated. All patients underwent colectomy due to severe disease, resistant to conservative treatment. Inflammation activity was determined according to severity of erosive and ulcerative lesions of the colon. Growth factor and chemokine levels in supernatant fluid after centrifugal separation of homogenate of 10 mg biopsy specimen were investigated by the protein analyzer «Bio-Plex» («Bio-Rad», USA). Biopsy specimens of uninvolved colonic mucosa resected in 10 cancer patients were used as a control. Results. Growth factor and chemokine levels were significantly elevated in colonic biopsy specimens of UC patients in comparison to that of controls: G-CSF 52,5±17,0 and 0,4±0,1 pg/10 mg (p<0,01), GM-CSF6,8±1,0 and 2,7±0,26 pg/10 mg (p <0,001), MCP-1 26,0 ±11,4 and 1,5±0,15 pg/10 mg (p <0,05), MIP-1β 12,5±2,8 and 3,2±0,4 pg/10 mg (p <0,01) respectively. The content of G-CSF, MCP-1 and MIP-1β in colonic biopsy specimens was increased in comparison to their level in non-involved terminal ileum biopsy specimens. Levels in the left part of the colon and transverse colon were higher, than in the cecum and ascending colon. Growth factor and chemokine concentrations were significantly higher at severe inflammation in comparison to moderate inflammatory activity: G-CSF - 25 fold, MCP-1 - 6,4 fold, MIP-1β -3 fold and GM-CSF - 2,6 fold. Profiles GM-CSF > G-CSF and MIP-1β> MCP-1 were observed in 22 (95,7%) and 19 (82,6%) of 23 colonic biopsy specimens of the control group respectively, profiles G-CSF > GM-CSF and MCP-1> MIP-1β - in 17 (89,4%) and 12 (63,1%) of 19 colonic biopsies of UC patients with severe inflammatory activity. Conclusions. Significant increase in neutrophil growth factor level G-CSF and monocyte chemokine MCP-1 was observed in colonic biopsy specimens of UC patients at widespread destructive and inflammatory ulcerative process.
About the Authors
Yevgeny A. Konovich
Federal government-financed institution «Ryzhikh State Scientific Center of Coloproctology»
Russian Federation
Russian Federation
K. Ye. Shirokikh
Federal government-financed institution «Ryzhikh State Scientific Center of Coloproctology»
Russian Federation
Russian Federation
M. V. Shapina
Federal government-financed institution «Ryzhikh State Scientific Center of Coloproctology»
Russian Federation
Russian Federation
Igor L. Khalif
Federal government-financed institution «Ryzhikh State Scientific Center of Coloproctology»
Russian Federation
Russian Federation
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Review
For citations:
Konovich Ye.A., Shirokikh K.Ye., Shapina M.V., Khalif I.L. Colonic growth factors (G-CSF, GM-CSF) and chemokines (MCP-1, MIP-1β) in severe ulcerative colitis. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2016;26(5):74-81. (In Russ.) https://doi.org/10.22416/1382-4376-2016-5-74-81
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