The role of cytokine genetic polymorphism in development of acute pancreatitis: analysis of intergenic and environmental interactions
https://doi.org/10.22416/1382-4376-2017-27-3-27-33
Abstract
Aim of investigation. To study association interleukin-1 (IL-1) gene (rs16944), interleukin-6 (IL-6) gene (rs1800795), interleukin-10 (IL-10) gene (rs1800896) polymorphisms with development of acute pancreatitis (AP) in the Russian population. Material and methods. Whole blood samples were received from 297 AP patients and 238 healthy controls. Genotyping of IL-1 gene (rs16944) polymorphisms, IL-6 gene (rs1800795), IL-10 gene (rs1800896) was carried out by polymerase chain reaction with allele discrimination by TaqMan-probes. Results. The genetic polymorphism combination 511СТ×174GC of IL-1 and IL-6 genes was associated to high risk of AP development (OR=2.25, 95%-CI 1.45-3.49; p=0.0018). According to stratification analysis smoking patients with 511CT genotype had higher AP risk, then the patients with other genotypes (OR=2.22, 95%-CI 1.3-3.79; p=0.003). Paired combination of genotypes to disease risk analysis demonstrated that at 511СT×174GС genotype combination the AP risk is highest at alcohol abuse history for over 10 years (OR=2.88, 95%-CI 1.59-5.23; p=0.0004). Conclusion. Interleukin genetic polymorphism investigation may be useful at assessment of cytokine status in AP patients to predict the outcomes and to develop the personalized approach to treatment and prophylaxis.
About the Authors
T. A. Samgina
Federal state educational government-financed institution of higher education «Kursk state medical university»
Russian Federation
Russian Federation
G. A. Zhivotova
Federal state educational government-financed institution of higher education «Kursk state medical university»
Russian Federation
Russian Federation
P. M. Nazarenko
Federal state educational government-financed institution of higher education «Kursk state medical university»
Russian Federation
Russian Federation
A. V. Polonikov
Federal state educational government-financed institution of higher education «Kursk state medical university»
Russian Federation
Russian Federation
References
1. Горский В.А., Ковальчук Л.В., Агапов М.А. Антимедиаторная терапия в комплексном лечении острого деструктивного панкреатита. Хирургия 2010;3:54-61.
2. La Rusch J., Whitcomb D.C. Genetics of pancreatitis. Curr Opin Gastroenterol 2011;27:467-74.
3. Curley P.J., McMahon M.J., Lancaster F. et al. Reduction in circulating levels of CD4 positive lymphocytes in acute pancreatitis: relationship to endotoxin, interleukin-6 and disease severity. Br J Surg 1993; 80:1312-5.
4. Yin Y.W., Sun Q.Q., Feng J.Q., Hu A.M., Liu H.L., Wang Q. Influence of interleukin gene polymorphisms on development of acute pancreatitis: asystematic review and meta-analysis. Mol Biol Rep 2013 Oct;40(10):5931-41.
5. Багненко С.Ф., Гольцов В.Р. Острый панкреатит: современное состояние проблемы и нерешённые вопросы. Альманах Института хирургии им. А.В.Вишневского 2008; 3(3):104-12
6. Синенченко Г.И., Пивоварова Л.П., Шиляев А.В., Двойнов В.Г. Прогностические критерии тяжести острого деструктивного панкреатита. Вестн Рос военно-мед акад 2007; 1(17): 100-5.
7. Biffi W., Moore E., Moore F., Pelerson V. lnterleukin-6 in the injured patient. Ann Surg 1996; 224:647-64.
8. Castell J.V., Gomes-Lechon M.J., David M. et al. Interleukin-6 is the major regulator of acute phase protein in synthesis in adult human hepatocytes. FEBS Lett 1989;242: 237-9.
9. Opal S., De Palo V. Anti-inflammatory cytokines. Chest 2000; 1(17):1162-72.
10. Heath D., Cruickshank A., Gudgeon M., Jehanli A. Shenkin A., Imrie C. Role of interleukin-6 in mediating the acute phase protein response potential as early means of severity assessment in acute pancreatitis. Gut 1993; 34(1):41-5.
11. Chi D.Z., Chen J., Huang D.P. Influence of interleukin-1β and interleukin-6 gene polymorphisms on the development of acute pancreatitis. Gen Mol Res 2015 Feb 3;14(1):975-80.
12. Liu Y., Dan G., Wu L. et al. Functional effect of polymorphisms in the promoter of TNFAIP3 (A20) in acute pancreatitis in the Han Chinese population. PLoS One 2014 Jul 22; 9(7):103-4.
13. Banks P.A., Bollen T.L., Dervenis C., Gooszen H.G. et al. Acute Pancreatitis Classification Working Group. Classification of acute pancreatitis 2012: revision of the Atlanta classification and definitions by international consensus. Gut 2013;62(1): 102-11.
14. Furtwaengler N.A., de Visser R.O. Lack of international consensus in low-risk drinking guidelines. Drug Alcohol Rev 2013;32:11-8.
15. Koressar T., Remm M. Enhancements and modifications of primer design program Primer3. Bioinformatics 2007;23:1289-91.
16. Симбирцев А.С. Биология семейства интерлейкин-1 человека. Иммунология 1998; 3:9-17.
17. Smithies A.M., Sargen K., Demaine A.G., King snorth A.N. Investigation of the interleukin 1 gene cluster and its association with acute pancreatitis. Pancreas 2000;20:234-40.
18. Powell J.J., Fearon K.C., Siriwardena A.K., Ross J.A. Evidence against a role for polymorphisms at tumor necrosis factor, interleukin-1 and interleukin-1 receptor antagonist gene loci in the regulation of disease severity in acute pancreatitis. Surgery 2001; 129:633-40.
19. Zhang D.L., Zheng H.M., Yu B.J., Jiang Z.W., Li J.S. Association of polymorphisms of IL and CD14 genes with acute severe pancreatitis and septic shock. World J Gastroenterol 2005;11:4409-13.
20. Chen X.Q. A study on the association between the interleukin-1b, interleukin-6 gene polymorphisms and the condition of patients with acute pancreatitis. Fujian Medical University (Master thesis); 2007. P. 1-49.
21. Tischendorf J.J., Yagmur E., Scholten D. et al. The interleukin-6 (IL-6)-174 G/C promoter genotype is associated with the presence of septic shock and the ex vivo secretion of IL-6. Int J Immunogenet 2007 Dec; 34(6):413-8.
22. Ferri C., Sciacca F.L., Grimaldi L.M.E. et al. Lack of association between IL-1A and IL-1B promoter polymorphisms and multiple sclerosis. J Neurol Neurosurg Psychiatr 2000; 69:564-5.
23. Furuta T., el-Omar E.M., Xiao F. et al. Interleukin 1 beta polymorphisms increase risk of hypochlorhydria and atrophic gastritis and reduce risk of duodenal ulcer recurrence in Japan. Gastroenterology 2002; 123(1):92105.
Review
For citations:
Samgina T.A., Zhivotova G.A., Nazarenko P.M., Polonikov A.V. The role of cytokine genetic polymorphism in development of acute pancreatitis: analysis of intergenic and environmental interactions. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2017;27(3):27-33. (In Russ.) https://doi.org/10.22416/1382-4376-2017-27-3-27-33
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