Microbiota of the Esophagus and Stomach in Patients with Gastroesophageal Reflux Disease and Healthy Volunteers
https://doi.org/10.22416/1382-4376-2018-28-4-36-46
Abstract
This paper is aimed at investigating the microbiota of the esophagus and stomach in patients with gastroesophageal reflux disease (GERD) and healthy volunteers.
Materials and methods. The study included 15 patients suffering from GERD and 6 healthy volunteers. All subjects underwent sampling of esophageal and gastric contents. The study of the microbiota in the obtained samples was performed by sequencing the 16S gene of ribosomal RNA (rRNA).
Results. The most common types of bacteria in the esophagus and stomach in patients with GERD and healthy volunteers are found to be Bacteroidetes, Firmicutes, Actinobacteria, Proteobacteria, Fusobacterium. By comparing the relative contents of the main types of bacteria in the esophageal mucus and gastric contents, a significant decrease in the proportion of Proteobacteria was observed in patients with GERD as compared to healthy volunteers. The decrease in the relative number of bacteria belonging to the Acetobacteraceae, Bacillaceae, Bdellovibrionaceae, Clostridiales Insertae Sedis XI, Fusobacteriaceae, Moraxellaceae, Pasteurellaceae and Rhodocyclaceae families was observed in the esophagus in patients with GERD as compared to healthy volunteers. A higher bacterial content of the Leptotrichiaceae and Veillonellaceae families was detected in the stomach of patients with GERD.
Conclusions. The obtained results indicate differences in the intraluminal microbiota of the esophagus and stomach in patients with GERD and healthy volunteers. Further study should be carried out to study the effect of changes in bacterial composition on those in the esophagus and stomach.
About the Authors
D. E. Rumyantseva
I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Healthcare of the Russian Federation
Russian Federation
Physician of the Gastroenterology Department, Vasilenko Clinic of Internal Disease Propedeutics, Gastroenterology and Hepatology
Russian Federation
Physician of the Gastroenterology Department, Vasilenko Clinic of Internal Disease Propedeutics, Gastroenterology and Hepatology
A. S. Trukhmanov
I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Healthcare of the Russian Federation
Russian Federation
Dr. Sci. (Med), Professor of the Department of Propaedeutics of Internal Diseases
Russian Federation
Dr. Sci. (Med), Professor of the Department of Propaedeutics of Internal Diseases
A. V. Kudryavtseva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
Cand. Sci. (Biology), Head of the Laboratory of Post-Genomic Studies,
Research Associate of Pathological Anatomy Department, National Medical Research Radiological Centre
G. S. Krasnov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Russian Federation
Senior Researcher
Russian Federation
Senior Researcher
A. V. Paraskevova
I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Healthcare of the Russian Federation
Russian Federation
Physician of the Department of Functional Diagnostics, Vasilenko Clinic of Internal Disease Propedeutics, Gastroenterology and Hepatology
Russian Federation
Physician of the Department of Functional Diagnostics, Vasilenko Clinic of Internal Disease Propedeutics, Gastroenterology and Hepatology
O. A. Storonova
I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Healthcare of the Russian Federation
Russian Federation
Cand. Sci. (Med.), Physician of the Department of Functional Diagnostics, Vasilenko Clinic of Internal Disease Propedeutics, Gastroenterology and Hepatology
Russian Federation
Cand. Sci. (Med.), Physician of the Department of Functional Diagnostics, Vasilenko Clinic of Internal Disease Propedeutics, Gastroenterology and Hepatology
A. B. Ponomarev
I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Healthcare of the Russian Federation
Russian Federation
Cand. Sci. (Med.), Associate Professor of Pathological Anatomy Department
Russian Federation
Cand. Sci. (Med.), Associate Professor of Pathological Anatomy Department
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Review
For citations:
Rumyantseva D.E., Trukhmanov A.S., Kudryavtseva A.V., Krasnov G.S., Paraskevova A.V., Storonova O.A., Ponomarev A.B. Microbiota of the Esophagus and Stomach in Patients with Gastroesophageal Reflux Disease and Healthy Volunteers. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2018;28(4):36-46. (In Russ.) https://doi.org/10.22416/1382-4376-2018-28-4-36-46
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