Genetic polymorphism of xenobiotic biotransformation genes, TNF-α and IL-4 in obstructive jaundice
Abstract
Aim of investigation. To study polymorphism of СYP2E1-1293 G/C (c1/c2), CYP 3А4 1А/1В, NAT2 Leu161Leu (481 c/t), GSTP1 Ile105Val, IL4 C-589T, TNF-α G-308A genes at syndrome of obstructive jaundice and uncomplicated gallstone disease.
Material and methods. Overall 54 patients were divided into three comparison groups: those with obstructive jaundice of benign etiology, with cholestasis due to malignancy and uncomplicated gallstone disease. Genomic DNA of patients obtained from blood leukocytes analysed with application of «DNK-ekspresskrov» reagent utilizing «SNP-ekspress-RV» device of LLC Research and production company «Litekh» (Moscow).
Results. Carriage of C allele of СYP2E1 gene is a risk factor of development of biliary diseases. The interrelation of A allele of TNF-α gene G-308A (OR=2,68, 95% CI 1,23-5,84) with high risk of obstructive jaundice due to choledocholithiasis was revealed. At the same time carriage of Т-allele of NAT2 Leu161Leu and IL4 C-589T genes reduces risk of obstructive jaundice of benign etiology.
Conclusion. The high risk of biliary diseases is associated to allele C of СYP2E1-1293 G/C gene. Carriers of A allele of TNF-α gene G-308A have higher risk of obstructive jaundice on a background of gallstone disease, while Т alleles of NAT2 Leu161Leu and IL4 C-589T genes have protective action for posthepatic cholestasis.
About the Authors
A. A. Natalsky
Pavlov Ryazan State Medical University, Ministry of heathcare of the Russian Federation
Russian Federation
Russian Federation
Natalsky Aleksandr A — MD, assistant-professor, Chair of hospital course of surgery
390026, Ryazan, Vysokovoltnaya street, 9.
S. V. Tarasenko
Pavlov Ryazan State Medical University, Ministry of heathcare of the Russian Federation
Russian Federation
Russian Federation
A. A. Nikiforov
Pavlov Ryazan State Medical University, Ministry of heathcare of the Russian Federation
Russian Federation
Russian Federation
O. V. Zaytsev
Pavlov Ryazan State Medical University, Ministry of heathcare of the Russian Federation
Russian Federation
Russian Federation
O. D. Peskov
Pavlov Ryazan State Medical University, Ministry of heathcare of the Russian Federation
Russian Federation
Russian Federation
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Review
For citations:
Natalsky A.A., Tarasenko S.V., Nikiforov A.A., Zaytsev O.V., Peskov O.D. Genetic polymorphism of xenobiotic biotransformation genes, TNF-α and IL-4 in obstructive jaundice. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2015;25(4):23-28. (In Russ.)
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