Electrical parameters and structure of membranes of erythrocytes at diffuse liver diseases
Abstract
Aim of investigation. To compare electrical char- acteristics of erythrocytes to structural changes of their membranes at diffuse liver disease (DLD).
Materials and methods. One hundred and thirty men 35–60 year old with DLD (chronic hepatitis and cirrhosis) and 33 practically healthy persons of compa- rable age were investigated. Parameters of red blood cells were assessed by standard methods, structure of erythrocyte membranes was investigated by thin- layer chromatography method, electrical properties by dielectrophoresis method in heterogeneous variable electrical field in 0,05·106–1·106 Hz frequency range.
Results. In DLD patients electrical conduction of membrane, scores of aggregation, destruction, capac- ity of membranes of erythrocytes is higher, and migra- tion speed of cells to electrodes – is lower, than in control (р < 0,0001–0,05). These disorders grow along with severity of disease and are intimately linked to changes of lipid composition of erythrocyte membranes – increase of cholesterol fraction relative content with decrease of level of total lipids, relative content of phos- pholipids (PL), triglycerides and cholesterol aethers (р < 0,002–0,05). Cholesterol to PL ratio grows due to decrease of total PLs level and increase of PL lysofrac- tions relative content. The score of erythrocyte destruc- tion grows respectively.
Conclusions. Obtained changes of erythrocyte electrical characteristics and structural components of their membranes can be used for evaluation of severity of diffuse liver diseases and assessment of treatment efficacy.
About the Authors
S. A. KurilovichRussian Federation
M. V. Kruchinina
Russian Federation
V. M. Generalov
Russian Federation
T. S. Bakirov
Russian Federation
V. A. Rikhter
Russian Federation
D. V. Semenov
Russian Federation
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Review
For citations:
Kurilovich S.A., Kruchinina M.V., Generalov V.M., Bakirov T.S., Rikhter V.A., Semenov D.V. Electrical parameters and structure of membranes of erythrocytes at diffuse liver diseases. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2009;19(2):30-36. (In Russ.)
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