The clinical role of blood coagulation and platelet receptors gene allelic variants in development of cryoglobulinemic vasculitis at chronic hepatitis C

Aim of investigation. To estimate the clinical and prognostic value of carriage of various blood coagulation and platelet receptors gene allelic variants in development of cryoglobulinemic vasculitis at chronic hepatitis C (CHC). Material and methods. Original study included overall 200 patients with CHCs and liver cirrhosis in its outcome, who were divided into 3 groups: patients without cryoglobulinemia (CG, n=123), those with asymptomatic cryoglobulinemia (ACG, n=40) and with cryoglobulinemic vasculitis (CGV, n=37). Assessment of polymorphism of the studied genes was carried out by real-time polymerase chain reaction with melting curve analysis. Results. CGV patients in comparison to those with ACG had 4G mutant allele are significantly more frequent (odds ratio (OR): 4G=2,008) as well as genotypes 5G4G+4G4G (OR: 5G4G+4G4G=4,950) of the gene PAI-675 5G/4G, and in comparison to patients without CG - CC mutant genotype of the gene ITGB3 1565 T/C (р =0,047). The multifactor analysis at comparison of patients with CGV and without it revealed the quantity of mutant alleles of PAI-675 5G/4G and ITGB3 1565 T/C genes and infection duration as independent factors for vasculitis development, while at comparison of patients with CGV and ACG - only quantity of mutant alleles of these genes. Patients with CGV allele C and CC homozygosity of the gene ITGB3 1565 T/C is associated to presence of renal diseases, while allele C genotypes (TC+CC) of ITGB3 1565 T/C gene is associated with involvement of muscular and nervous system. Conclusion. Carriage of mutant genotypes of PAI-675 5G/4G and ITGB3 1565 T/C genes is a factor which allows to predict CGV development in CHC patients, and can determine clinical manifestations of the latter.

хронический гепатит С, крио-глобулинемический васкулит, полиморфизм генов, тромбофилия, гемостаз, тромбоцитарные рецепто-ры

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