Quantitative dynamic evaluation HBsAg in blood in comparison to viral load level in patients with chronic HBV-monoinfection
Abstract
Aim of investigation. Quantitative analysis of HBsAg contents in the blood in relation to HBV viremia level in patients with various variants of natural course of chronic HBV-infection at dynamic evaluation within 1,5–2 years.
Material and methods. Overall 135 patients with chronic HBV-monoinfection were included in original study. Dynamic assessment of HBV viremia level by polymerase chain reaction and quantitative assessment of blood HBsAg by chemiluminescence was carried out.
Results. Blood HBsAg concentration significantly differed between НВе-positive and НВе-negative phases of chronic HBV-infection. At analysis of serum HBsAg fluctuations in НВе-negative chronic hepatitis B patients no correlation of HBsAg concentration with the level of HBV viremia was revealed at dynamic observation for 1,5 to 2 years. Received data on low HBsAg concentration in a part of inactive HBV carriers allow to consider, that quantitative assessment of HBsAg contents as a supplemental criterion for diagnosis of «hepatitis B virus inactive carriage».
About the Authors
K. R. DudinaRussian Federation
O. O. Znoyko
Russian Federation
S. A. Shutko
Russian Federation
Ye. A. Klimova
Russian Federation
L. Ye. Kuzina
Russian Federation
D. M. Braginsky
Russian Federation
N. P. Blokhina
Russian Federation
N. D. Yushchuk
Russian Federation
References
1. Brunetto M.R., Oliveri F., Colombatto P. Hepatitis B surface antigen serum levels help to distinguish active from inactive hepatitis B virus genotype D carriers // Gastroenterology. – 2010. – Vol. 139, N 2. – P. 483–490.
2. Chen C.H., Lee C.M., Wang J.H. Correlation of quantitative assay of hepatitis B surface antigen and HBV DNA levels in asymptomatic hepatitis B virus carriers // Eur. J. Gastroenterol. Hepatol. – 2004. – Vol. 16, N 11. – P. 1213–1218.
3. Deguchi M., Yamashita N., Kagita M. et al. Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay
4. // J. Virol. Methods. – 2004. – Vol. 115. – P. 217–222.
5. Jaroszewicz J., Serrano B.С., Deterding K. Hepatits B surface antigen (HBsAg) levels in the natural history of hepatitis B virus (HBV)-infection: a European perspective // J. Hepatol. – 2010. – Vol. 52, N 4. – P. 514–522.
6. Jaroszewicz J., Serrano B.C., Deterding K. et al. HBsAg serum levels are associated with the phase of HBVinfection // J. Hepatol. – 2009. – Vol. 50 (suppl. 1). – P. 139. – Abstract: 364.
7. Kuhns M.C., Kleinman S.H., McNamara A.L. The REDS Study Group. Lack of correlation between HBsAg and HBV DNA levels in blood donors who test positive for HBsAg and anti-HBc: implications for future HBV screening policy // Transfusion. – 2004. – Vol. 44, N 9. – P. 1332–1339.
8. Lei J.H., Yang X., Luo H.Y. Serum HBsAg concentration and HBV replication level in hepatitis B patients with positive serum HBsAg and HBeAg // Zhong Nan Da Xue Xue Bao Yi Xue Ban. – 2006. – Vol. 31, N 4. – P. 548–551.
9. Lu L., Ye D.-W., Wang Y.-D. Relationship between HBV cccDNA and HBsAg levels is associated with HBeAg statuses of chronic hepatitis B patients // J. Hepatol.
10. – 2009. – Vol. 50 (suppl. 1). – P. 209. – Abstract: 567.
11. Mühlbacher A., Weber B., Bürgisser P. Multicenter study of a new fully automated HBsAg screening assay with enhanced sensitivity for the detection of HBV mutants // Med. Microbiol. Immunol. – 2008. – Vol. 197. – P. 55–64.
12. Nguyen T., Thompson A.J., Bowden S. Hepatitis B surface antigen levels during the natural history of chronic hepatitis B: a prospective on Asia // J. Hepatol. – 2010. – Vol. 52, N 4. – P. 508–513.
13. Ozdil B., Cosar A.M., Akkiz H. Negative correlation between viral load and HBsAg levels in chronic HBVinfected patients // Arch. Virol. – 2009. – Vol. 154, N 9. – P. 1451–1455.
14. Rodella A., Galli C., Terlenghi L. et al. Quantitative analysis of HBsAg, IgM anti-HBc and anti-HBc avidity in acute and chronic hepatitis B // J. Clin. Virol. – 2006. – Vol. 37, N 3. – P. 206–212.
15. Su T.H., Hsu C.S., Chen C.L. Serum hepatitis B surface antigen concentration correlates with HBV DNA level in patients with chronic hepatitis B // Antivir. Ther. – 2010. – Vol. 15, N 8. – P. 1133–1139.
16. Thompson A.J., Nguyen T., Iser D. Serum hepatitis B surface antigen and hepatitis B e antigen titers: disease phase influences correlation with viral load and intrahepatic hepatitis B virus markers // Hepatology. – 2010. – Vol. 51, N 6. – P. 1933–1944.
17. Villar L.M., Medina H., Villela-Nogueira C.A. et al. Saliva as a source for hepatitis B virus diagnosis // J. Hepatol. – 2010. – Vol. 52 (suppl. 1). – P. 248. – Abstract: 634.
18. Wang G.S., Wang M.M., Xie Q.L. Expression and clinical significance of HBsAg and HBcAg in hepatocytes in chronic hepatitis B // Zhonghua Gan Zang Bing Za Zhi. – 2004. – Vol. 12, N 5. – P. 287–289.
19. Wiegand J., Wedemeyer H., Finger A. A decline in hepatitis B virus surface antigen (HBsAg) predicts clearance, but does not correlate with quantitative hbeag or HBV DNA levels // Antivir. Ther. – 2008. – Vol. 13, N 4. – P. 547–554.
Review
For citations:
Dudina K.R., Znoyko O.O., Shutko S.A., Klimova Ye.A., Kuzina L.Ye., Braginsky D.M., Blokhina N.P., Yushchuk N.D. Quantitative dynamic evaluation HBsAg in blood in comparison to viral load level in patients with chronic HBV-monoinfection. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2011;21(4):37-42. (In Russ.)
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