The effect of nuclear transcriptional factor κВ activation inhibitor – curcumine on the experimental immune liver disease
Abstract
Aim of investigation. To study at two models of immune hepatitis (Т-cellular and antibody origin) in mice damage of the liver, activity and destruction of its immunocompetent cells, thymus gland and the spleen, as well as the effect of curcumine — inhibitor of NF-κB activation on development of pathological process.
Material and methods. An immune lesion of the liver in mice of СВА line was invoked by Т-cellular mitogen concanavalin A and xenogenic anti-liver antibodies. Study was carried out with application of histological, cytological and biochemical methods.
Results. In comparative studies features of hepatitis development of cellular and antibody origin were revealed. At Т-cellular hepatitis cytolytic component of liver lesion, primary and secondary cell necrosis, depression of cytophagous activity of macrophages that causes preconditions for enhancement of inflammation were more intensive. At hepatitis of antibody origin, at least at early stages, vascular and immunological reactions are expressed in the liver.
Conclusion. Curcumine, the inhibitor of NF-κB activation reduces preconditions for development of inflammatory response at experimental Т-cellular hepatitis, and has protective action for the liver.
About the Authors
I. N. Alekseyeva
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
N. V. Makogon
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
S. I. Pavlovich
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
T. M. Bryzgina
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
L. I. Aleksyuk
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
T. V. Martynova
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
V. S. Sukhina
Институт физиологии им. А.А. Богомольца НАН Украины
Ukraine
Ukraine
References
1. Алексеева И.Н. Противопеченочные антитела и функции печени. – Киев: Наукова думка, 1980. – 184 с.
2. Камышников В.С. Справочник по клинико-биохимическим исследованиям и лабораторной диагностике. – М.: МЕДпресс-информ, 2004. – 912 с.
3. Лакин Г.Ф. Биометрия. – М.: Высшая школа, 1980. – 293 с.
4. Макогон Н.В., Павлович С.І., Бризгiна Т.М. и др. Пролiферацiя та загибель мононуклеарних клiтин печiнки мишей за умов її iмунного ураження, викликаного введенням конканавалiну А або протипечiнкових антитiл // Фiзiол. журн. – 2008. – Т. 54, № 6. – С. 49–57.
5. Мартинова Т.В., Алексєєва І.М. Функцiональна активнiсть перитонеальних макрофагiв у мишей при iмунному ушкодженнi печiнки клiтинного та антитiльного генезу // Фiзiол. журн. – 2008. – Т. 55, № 1. – С. 36–42.
6. Cuzzocrea S., Chatterjee P.K., Mazzon E. et al. Pyrrolidine dithiocarbamate attenuates the development of acute and chronic inflammation // Br. J. Pharm. – 2002. – Vol. 135, N 2. – Р. 496–510.
7. Czaja A. Autoantibodies in autoimmune liver disease // Adv. Clin. Chem. – 2005. – Vol. 40. – Р. 127–164.
8. Czaja A. Current concepts in autoimmune hepatitis // Ann. Hepatol. – 2005. – Vol. 4, N 1. – Р. 6–24.
9. Dong Z.J., Wei H.M., Sun R. et al. Isolation of murine hepatic lymphocytes using mechanical dissection for phenotypic and functional analysis of NK1.1+ cells // World J. Gastroenterol. – 2004. – Vol. 10, N 13. – Р. 1928–1933.
10. Gao X., Kuo J., Jiang H. et al. Immunomodulatory activity of curcumin: suppression of limphocyte proliferation, development of cell-mediated cytotoxicity, and cytokine production in vitro // Biochem. Pharm. – 2004. – Vol. 68. – Р. 51–61.
11. Jiang H., Deng C-S., Zhang M., Xia J. Curcuminattenuated trinitrobenzene sulphonic acid induces chronic colitis by inhibiting expression of cyclooxygenase-2 // World J. Gastroenterol. – 2006. – Vol. 12, N 24. – Р. 3848 – 3853.
12. Kang B., Song Y., Kim K. et al. Curcumin inhibits Th1 cytokine profile in CD4+ T cells by suppressing interleukin-12 production in macrophages // British J. Pharm. – 1999. – Vol. 128, N 2. – Р. 380–384.
13. Koerber K., Sass G., Kiemer A.K. et al. In vivo regulation of inducible NO synthase in immune-mediated liver injury in mice // Hepatology. – 2002. – Vol. 36, N 5. – Р. 1061–1069.
14. Leclercq I.A., Farrell G.C., Sempoux C. et al. Curcumin inhibits NF-kB activation and reduces the severity of experimental steatohepatitis in mice // J. Hepatol. – 2004. – Vol. 41, N 6. – Р. 926–934.
15. Luedde T., Trautwein C. Intracellular survival pathways in the liver // Liver International. – 2006. – Vol. 26. – Р. 1163–1174.
16. Mackay I.R. Hepatoimmunology: a perspective // Immunol. Cell Biol. – 2002. – Vol. 80, N 1. – Р. 36– 44.
17. Reyes-Gordillo K., Segovia J., Shibayama M. et al. Curcumin protects against acute liver damage in the rat by inhibiting NF-kB proinflammatory cytokines production and oxidative stress // Biochem. Biophys. Acta. – 2007. – Vol. 1770. – Р. 989–996.
18. Shen S-Q., Zhang Y., Xiang J-J. et al. Protective effect of curcumin against liver warm ischemia/reperfusion injury in rat model is associated with regulation of heat shock protein and antioxidant enzymes // World J. Gastroenterol. – 2007. – Vol. 13, N 13. – Р. 1953– 1961.
19. Shimizu S., Eguchi Y., Kamiike W. et al. Involvement of ICE family proteases in apoptosis induced by reoxygenation of hypoxic hepatocytes // Am. J. Phisiol. – 1996. – Vol. 271, N 6. – Р. 949–958.
20. Tiegs G. Cellular and cytokine-mediated mechanisms of inflammation and its modulation in immune-mediated liver injury // Z. Gastroenterol. – 2007. – Vol. 45, N 1. – Р. 63–70.
21. Tiegs G., Hentschel G., Wendel A. A T cell-dependent experimental liver injury in mice inducible by Concanavalin A // J. Clin. Invest. – 1992. – Vol. 90, N 1. – Р. 196–203.
22. Washington M.K. Autoimmune liver disease: overlap and outliers // Modern Pathology. – 2007. – Vol. 20, N 6. – Р. 15–30.
23. Wullaert A., van Loo G., Heyninck., Beyaert R. Hepatic tumor necrosis factor signaling and nuclear factor-kB: effects on liver homeostasis and beyond // Endocrine Reviews. – 2007. – Vol. 28, N 4. – Р. 365–386.
Review
For citations:
Alekseyeva I.N., Makogon N.V., Pavlovich S.I., Bryzgina T.M., Aleksyuk L.I., Martynova T.V., Sukhina V.S. The effect of nuclear transcriptional factor κВ activation inhibitor – curcumine on the experimental immune liver disease. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2010;20(1):38-43. (In Russ.)
Views:
86
Email this article 
